Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

4ZTT

Crystal structures of ferritin mutants reveal diferric-peroxo intermediates

これはPDB形式変換不可エントリーです。
4ZTT の概要
エントリーDOI10.2210/pdb4ztt/pdb
分子名称ferritin, OXYGEN ATOM, MU-OXO-DIIRON, ... (11 entities in total)
機能のキーワードhelicobactor pylori, escherichia coli, gram-negative bacteria, ferritin, iron uptake, ferroxidase center, reaction intermediate, oxidoreductase
由来する生物種Escherichia coli DH1
詳細
タンパク質・核酸の鎖数6
化学式量合計119741.50
構造登録者
Kim, S.,Park, Y.H.,Jung, S.W.,Seok, J.H.,Chung, Y.B.,Lee, D.B.,Gowda, G.,Lee, J.H.,Han, H.R.,Cho, A.E.,Lee, C.,Chung, M.S.,Kim, K.H. (登録日: 2015-05-15, 公開日: 2016-06-15, 最終更新日: 2024-10-02)
主引用文献Kim, S.,Lee, J.H.,Seok, J.H.,Park, Y.H.,Jung, S.W.,Cho, A.E.,Lee, C.,Chung, M.S.,Kim, K.H.
Structural Basis of Novel Iron-Uptake Route and Reaction Intermediates in Ferritins from Gram-Negative Bacteria.
J. Mol. Biol., 428:5007-5018, 2016
Cited by
PubMed Abstract: Iron and oxygen chemistry is mediated by iron proteins for many biological functions. Carboxylate-bridged diiron enzymes including ferritin have the common mechanism of oxygen activation via peroxodiferric intermediates. However, the route for iron uptake and the structural identification of intermediates still remain incomplete. The 4-fold symmetry channel of Helicobacter pylori ferritin was previously proposed as the iron-uptake route in eubacteria, but the amino acid residues at the 4-fold channel are not highly conserved. Here, we show evidence for a short path for iron uptake from His93 on the surface to the ferroxidase center in H. pylori ferritin and Escherichia coli ferritin. The amino acid residues along this path are highly conserved in Gram-negative bacteria and some archaea, and the mutants containing S20A and H93L showed significantly decreased iron oxidation. Surprisingly, the E. coli ferritin S20A crystal structure showed oxygen binding and side-on, symmetric μ-η:η peroxodiferric and oxodiferric intermediates. The results provide the structural basis for understanding the chemical nature of intermediates in iron oxidation in bacteria and some of archaea.
PubMed: 27777002
DOI: 10.1016/j.jmb.2016.10.022
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.83 Å)
構造検証レポート
Validation report summary of 4ztt
検証レポート(詳細版)ダウンロードをダウンロード

239149

件を2025-07-23に公開中

PDB statisticsPDBj update infoContact PDBjnumon