4ZSX

Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-2.0

4ZSX の概要

• エントリーDOI: 10.2210/pdb4zsx/pdb
• 関連するPDBエントリー: 4ZSV 4ZSZ
• 分子名称: Uncharacterized Fusion Protein, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: protein design, bionanotechnology, protein assembly, symmetry, biomaterials, unknown function
• 由来する生物種: Aquifex aeolicus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68772.27

構造登録者

Lai, Y.-T.,Yeates, T.O. (登録日: 2015-05-14, 公開日: 2015-08-19, 最終更新日: 2023-09-27)

主引用文献

Lai, Y.T.,Jiang, L.,Chen, W.,Yeates, T.O.
On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.
Protein Eng.Des.Sel., 28:491-500, 2015

PubMed Abstract: Connecting proteins together in prescribed geometric arrangements is an important element in new areas of biomolecular design. In this study, we characterize the degree of three-dimensional orientational control that can be achieved when two protein domains that have alpha-helical termini are joined using an alpha-helical linker. A fusion between naturally oligomeric protein domains was designed in this fashion with the intent of creating a self-assembling 12-subunit tetrahedral protein cage. While the designed fusion protein failed to assemble into a tetrahedral cage in high yield, a series of crystal structures showed that the two fused components were indeed bridged by an intact alpha helix, although the fusion protein was distorted from the intended ideal configuration by bending of the helix, ranging from 7 to 35°. That range of deviation in orientation creates challenges for designing large, perfectly symmetric protein assemblies, although it should offer useful outcomes for other less geometrically demanding applications in synthetic biology.

PubMed: 26243886
DOI: 10.1093/protein/gzv035

実験手法

X-RAY DIFFRACTION (2.19 Å)