4ZRA

CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LPRG BINDING TO TRIACYLGLYCERIDE

4ZRA の概要

• エントリーDOI: 10.2210/pdb4zra/pdb
• 関連するPDBエントリー: 3MH8 3MHA
• 分子名称: Lipoprotein LprG, Tripalmitoylglycerol (3 entities in total)
• 機能のキーワード: lprg, lipoprotein, structural genomics, tb structural genomics consortium, tbsgc, lipid binding protein
• 由来する生物種: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
• 細胞内の位置: Cell membrane ; Lipid-anchor : P9WK45
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43737.20

構造登録者

Martinot, A.J.,Farrow, M.,Bai, L.,Layre, E.,Cheng, T.Y.,Tsai, J.H.C.,Iqbal, J.,Annand, J.,Sullivan, Z.,Hussain, M.,Sacchettini, J.,Moody, D.B.,Seeliger, J.,Rubin, E.J.,TB Structural Genomics Consortium (TBSGC) (登録日: 2015-05-12, 公開日: 2016-02-10, 最終更新日: 2024-03-06)

主引用文献

Martinot, A.J.,Farrow, M.,Bai, L.,Layre, E.,Cheng, T.Y.,Tsai, J.H.,Iqbal, J.,Annand, J.W.,Sullivan, Z.A.,Hussain, M.M.,Sacchettini, J.,Moody, D.B.,Seeliger, J.C.,Rubin, E.J.
Mycobacterial Metabolic Syndrome: LprG and Rv1410 Regulate Triacylglyceride Levels, Growth Rate and Virulence in Mycobacterium tuberculosis.
Plos Pathog., 12:e1005351-e1005351, 2016

PubMed Abstract: Mycobacterium tuberculosis (Mtb) mutants lacking rv1411c, which encodes the lipoprotein LprG, and rv1410c, which encodes a putative efflux pump, are dramatically attenuated for growth in mice. Here we show that loss of LprG-Rv1410 in Mtb leads to intracellular triacylglyceride (TAG) accumulation, and overexpression of the locus increases the levels of TAG in the culture medium, demonstrating a role of this locus in TAG transport. LprG binds TAG within a large hydrophobic cleft and is sufficient to transfer TAG from donor to acceptor membranes. Further, LprG-Rv1410 is critical for broadly regulating bacterial growth and metabolism in vitro during carbon restriction and in vivo during infection of mice. The growth defect in mice is due to disrupted bacterial metabolism and occurs independently of key immune regulators. The in vivo essentiality of this locus suggests that this export system and other regulators of metabolism should be considered as targets for novel therapeutics.

PubMed: 26751071
DOI: 10.1371/journal.ppat.1005351

実験手法

X-RAY DIFFRACTION (1.83 Å)