4ZPY

Structure of N170A MVM mutant empty capsid

4ZPY の概要

• エントリーDOI: 10.2210/pdb4zpy/pdb
• 分子名称: VP1 protein (1 entity in total)
• 機能のキーワード: minute virus of mice, empty capsid, n170a mutant, virus
• 由来する生物種: Murine minute virus strain MVM prototype
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61203.05

構造登録者

Guerra, P.,Querol-Audi, J.,Silva, C.,Mateu, M.G.,Verdaguer, N. (登録日: 2015-05-08, 公開日: 2017-05-24, 最終更新日: 2024-01-10)

主引用文献

Guerra, P.,Valbuena, A.,Querol-Audi, J.,Silva, C.,Castellanos, M.,Rodriguez-Huete, A.,Garriga, D.,Mateu, M.G.,Verdaguer, N.
Structural basis for biologically relevant mechanical stiffening of a virus capsid by cavity-creating or spacefilling mutations.
Sci Rep, 7:4101-4101, 2017

PubMed Abstract: Recent studies reveal that the mechanical properties of virus particles may have been shaped by evolution to facilitate virus survival. Manipulation of the mechanical behavior of virus capsids is leading to a better understanding of viral infection, and to the development of virus-based nanoparticles with improved mechanical properties for nanotechnological applications. In the minute virus of mice (MVM), deleterious mutations around capsid pores involved in infection-related translocation events invariably increased local mechanical stiffness and interfered with pore-associated dynamics. To provide atomic-resolution insights into biologically relevant changes in virus capsid mechanics, we have determined by X-ray crystallography the structural effects of deleterious, mechanically stiffening mutations around the capsid pores. Data show that the cavity-creating N170A mutation at the pore wall does not induce any dramatic structural change around the pores, but instead generates subtle rearrangements that propagate throughout the capsid, resulting in a more compact, less flexible structure. Analysis of the spacefilling L172W mutation revealed the same relationship between increased stiffness and compacted capsid structure. Implications for understanding connections between virus mechanics, structure, dynamics and infectivity, and for engineering modified virus-based nanoparticles, are discussed.

PubMed: 28642465
DOI: 10.1038/s41598-017-04345-w

実験手法

X-RAY DIFFRACTION (3.8 Å)