4ZOR

The structure of the S37P MS2 viral capsid assembly.

4ZOR の概要

• エントリーDOI: 10.2210/pdb4zor/pdb
• 分子名称: Coat protein, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: capsid, virus like particle, virus
• 由来する生物種: Enterobacteria phage MS2
• 細胞内の位置: Virion : P03612
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 69122.39

構造登録者

Asensio, M.A.,Sankaran, B.,Zwart, P.H.,Tullman-Ercek, D. (登録日: 2015-05-06, 公開日: 2016-09-07, 最終更新日: 2023-09-27)

主引用文献

Asensio, M.A.,Morella, N.M.,Jakobson, C.M.,Hartman, E.C.,Glasgow, J.E.,Sankaran, B.,Zwart, P.H.,Tullman-Ercek, D.
A Selection for Assembly Reveals That a Single Amino Acid Mutant of the Bacteriophage MS2 Coat Protein Forms a Smaller Virus-like Particle.
Nano Lett., 16:5944-5950, 2016

PubMed Abstract: Virus-like particles are used to encapsulate drugs, imaging agents, enzymes, and other biologically active molecules in order to enhance their function. However, the size of most virus-like particles is inflexible, precluding the design of appropriately sized containers for different applications. Here, we describe a chromatographic selection for virus-like particle assembly. Using this selection, we identified a single amino acid substitution to the coat protein of bacteriophage MS2 that mediates a uniform switch in particle geometry from T = 3 to T = 1 icosahedral symmetry. The resulting smaller particle retains the ability to be disassembled and reassembled in vitro and to be chemically modified to load cargo into its interior cavity. The pair of 27 and 17 nm MS2 particles will allow direct examination of the effect of size on function in established applications of virus-like particles, including drug delivery and imaging.

PubMed: 27549001
DOI: 10.1021/acs.nanolett.6b02948

実験手法

X-RAY DIFFRACTION (2.2 Å)