4ZN2

Glycosyl hydrolase from Pseudomonas aeruginosa

Summary for 4ZN2

• Entry DOI: 10.2210/pdb4zn2/pdb
• Descriptor: PslG (2 entities in total)
• Functional Keywords: biofilm, exopolysaccharides, hydrolase
• Biological source: Pseudomonas aeruginosa PAO1
• Total number of polymer chains: 4
• Total formula weight: 189062.14

Authors

Su, T.,Liu, S.,Gu, L. (deposition date: 2015-05-04, release date: 2016-01-06, Last modification date: 2024-03-20)

Primary citation

Yu, S.,Su, T.,Wu, H.,Liu, S.,Wang, D.,Zhao, T.,Jin, Z.,Du, W.,Zhu, M.J.,Chua, S.L.,Yang, L.,Zhu, D.,Gu, L.,Ma, L.Z.
PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix
Cell Res., 25:1352-1367, 2015

PubMed Abstract: Biofilms are surface-associated communities of microorganism embedded in extracellular matrix. Exopolysaccharide is a critical component in the extracellular matrix that maintains biofilm architecture and protects resident biofilm bacteria from antimicrobials and host immune attack. However, self-produced factors that target the matrix exopolysaccharides, are still poorly understood. Here, we show that PslG, a protein involved in the synthesis of a key biofilm matrix exopolysaccharide Psl in Pseudomonas aeruginosa, prevents biofilm formation and disassembles existing biofilms within minutes at nanomolar concentrations when supplied exogenously. The crystal structure of PslG indicates the typical features of an endoglycosidase. PslG mainly disrupts the Psl matrix to disperse bacteria from biofilms. PslG treatment markedly enhances biofilm sensitivity to antibiotics and macrophage cells, resulting in improved biofilm clearance in a mouse implant infection model. Furthermore, PslG shows biofilm inhibition and disassembly activity against a wide range of Pseudomonas species, indicating its great potential in combating biofilm-related complications.

PubMed: 26611635
DOI: 10.1038/cr.2015.129

Experimental method

X-RAY DIFFRACTION (2 Å)