4ZN2

Glycosyl hydrolase from Pseudomonas aeruginosa

4ZN2 の概要

• エントリーDOI: 10.2210/pdb4zn2/pdb
• 分子名称: PslG (2 entities in total)
• 機能のキーワード: biofilm, exopolysaccharides, hydrolase
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 189062.14

構造登録者

Su, T.,Liu, S.,Gu, L. (登録日: 2015-05-04, 公開日: 2016-01-06, 最終更新日: 2024-03-20)

主引用文献

Yu, S.,Su, T.,Wu, H.,Liu, S.,Wang, D.,Zhao, T.,Jin, Z.,Du, W.,Zhu, M.J.,Chua, S.L.,Yang, L.,Zhu, D.,Gu, L.,Ma, L.Z.
PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix
Cell Res., 25:1352-1367, 2015

PubMed Abstract: Biofilms are surface-associated communities of microorganism embedded in extracellular matrix. Exopolysaccharide is a critical component in the extracellular matrix that maintains biofilm architecture and protects resident biofilm bacteria from antimicrobials and host immune attack. However, self-produced factors that target the matrix exopolysaccharides, are still poorly understood. Here, we show that PslG, a protein involved in the synthesis of a key biofilm matrix exopolysaccharide Psl in Pseudomonas aeruginosa, prevents biofilm formation and disassembles existing biofilms within minutes at nanomolar concentrations when supplied exogenously. The crystal structure of PslG indicates the typical features of an endoglycosidase. PslG mainly disrupts the Psl matrix to disperse bacteria from biofilms. PslG treatment markedly enhances biofilm sensitivity to antibiotics and macrophage cells, resulting in improved biofilm clearance in a mouse implant infection model. Furthermore, PslG shows biofilm inhibition and disassembly activity against a wide range of Pseudomonas species, indicating its great potential in combating biofilm-related complications.

PubMed: 26611635
DOI: 10.1038/cr.2015.129

実験手法

X-RAY DIFFRACTION (2 Å)