4ZM4

Complex structure of PctV K276R mutant with PMP and 3-dehydroshkimate

4ZM4 の概要

• エントリーDOI: 10.2210/pdb4zm4/pdb
• 関連するPDBエントリー: 4ZM3
• 分子名称: Aminotransferase, PYRIDOXAL-5'-PHOSPHATE, (3E,4R,5R)-4,5-dihydroxy-3-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}cyclohex-1-ene-1-carboxylic acid, ... (4 entities in total)
• 機能のキーワード: pyridoxal 5-phosphate, aminotransferase, transferase
• 由来する生物種: Streptomyces pactum
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 288799.82

構造登録者

Hirayama, A.,Miyanaga, A.,Kudo, F.,Eguchi, T. (登録日: 2015-05-02, 公開日: 2015-10-14, 最終更新日: 2023-11-08)

主引用文献

Hirayama, A.,Miyanaga, A.,Kudo, F.,Eguchi, T.
Mechanism-Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.
Chembiochem, 16:2484-2490, 2015

PubMed Abstract: Mutational analysis of the pyridoxal 5'-phosphate (PLP)-dependent enzyme PctV was carried out to elucidate the multi-step reaction mechanism for the formation of 3-aminobenzoate (3-ABA) from 3-dehydroshikimate (3-DSA). Introduction of mutation K276R led to the accumulation of a quinonoid intermediate with an absorption maximum at 580 nm after the reaction of pyridoxamine 5'-phosphate (PMP) with 3-DSA. The chemical structure of this intermediate was supported by X-ray crystallographic analysis of the complex formed between the K276R mutant and the quinonoid intermediate. These results clearly show that a quinonoid intermediate is involved in the formation of 3-ABA. They also indicate that Lys276 (in the active site of PctV) plays multiple roles, including acid/base catalysis during the dehydration reaction of the quinonoid intermediate.

PubMed: 26426567
DOI: 10.1002/cbic.201500426

実験手法

X-RAY DIFFRACTION (2.4 Å)