4ZKT

Crystal structure of the progenitor M complex of Clostridium botulinum type E neurotoxin

Summary for 4ZKT

• Entry DOI: 10.2210/pdb4zkt/pdb
• Descriptor: Bontoxilysin A, Botulinum neurotoxin type E, nontoxic-nonhemagglutinin component, NTNH, ZINC ION (3 entities in total)
• Functional Keywords: bont/e-ntnhe hetero-dimer, acidic cluster, domain swap, progenitor complex, hydrolase-toxin complex, hydrolase/toxin
• Biological source: Clostridium botulinum (strain Alaska E43 / Type E3); More
• Total number of polymer chains: 6
• Total formula weight: 843027.34

Authors

Eswaramoorthy, S.,Swaminathan, S. (deposition date: 2015-04-30, release date: 2015-12-23, Last modification date: 2024-10-23)

Primary citation

Eswaramoorthy, S.,Sun, J.,Li, H.,Singh, B.R.,Swaminathan, S.
Molecular Assembly of Clostridium botulinum progenitor M complex of type E.
Sci Rep, 5:17795-17795, 2015

PubMed Abstract: Clostridium botulinum neurotoxin (BoNT) is released as a progenitor complex, in association with a non-toxic-non-hemagglutinin protein (NTNH) and other associated proteins. We have determined the crystal structure of M type Progenitor complex of botulinum neurotoxin E [PTC-E(M)], a heterodimer of BoNT and NTNH. The crystal structure reveals that the complex exists as a tight, interlocked heterodimer of BoNT and NTNH. The crystal structure explains the mechanism of molecular assembly of the complex and reveals several acidic clusters at the interface responsible for association at low acidic pH and disassociation at basic/neutral pH. The similarity of the general architecture between the PTC-E(M) and the previously determined PTC-A(M) strongly suggests that the progenitor M complexes of all botulinum serotypes may have similar molecular arrangement, although the neurotoxins apparently can take very different conformation when they are released from the M complex.

PubMed: 26639353
DOI: 10.1038/srep17795

Experimental method

X-RAY DIFFRACTION (3.05 Å)