4ZKT

Crystal structure of the progenitor M complex of Clostridium botulinum type E neurotoxin

4ZKT の概要

• エントリーDOI: 10.2210/pdb4zkt/pdb
• 分子名称: Bontoxilysin A, Botulinum neurotoxin type E, nontoxic-nonhemagglutinin component, NTNH, ZINC ION (3 entities in total)
• 機能のキーワード: bont/e-ntnhe hetero-dimer, acidic cluster, domain swap, progenitor complex, hydrolase-toxin complex, hydrolase/toxin
• 由来する生物種: Clostridium botulinum (strain Alaska E43 / Type E3); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 843027.34

構造登録者

Eswaramoorthy, S.,Swaminathan, S. (登録日: 2015-04-30, 公開日: 2015-12-23, 最終更新日: 2024-10-23)

主引用文献

Eswaramoorthy, S.,Sun, J.,Li, H.,Singh, B.R.,Swaminathan, S.
Molecular Assembly of Clostridium botulinum progenitor M complex of type E.
Sci Rep, 5:17795-17795, 2015

PubMed Abstract: Clostridium botulinum neurotoxin (BoNT) is released as a progenitor complex, in association with a non-toxic-non-hemagglutinin protein (NTNH) and other associated proteins. We have determined the crystal structure of M type Progenitor complex of botulinum neurotoxin E [PTC-E(M)], a heterodimer of BoNT and NTNH. The crystal structure reveals that the complex exists as a tight, interlocked heterodimer of BoNT and NTNH. The crystal structure explains the mechanism of molecular assembly of the complex and reveals several acidic clusters at the interface responsible for association at low acidic pH and disassociation at basic/neutral pH. The similarity of the general architecture between the PTC-E(M) and the previously determined PTC-A(M) strongly suggests that the progenitor M complexes of all botulinum serotypes may have similar molecular arrangement, although the neurotoxins apparently can take very different conformation when they are released from the M complex.

PubMed: 26639353
DOI: 10.1038/srep17795

実験手法

X-RAY DIFFRACTION (3.05 Å)