4ZK9

The chemokine binding protein of orf virus complexed with CCL2

4ZK9 の概要

• エントリーDOI: 10.2210/pdb4zk9/pdb
• 関連するPDBエントリー: 4ZKB 4ZKC
• 分子名称: Chemokine binding protein, C-C motif chemokine 2, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: complex, orf virus chemokine binding protein, human c-c motif chemokine 2, viral protein-cytokine complex, viral protein/cytokine
• 由来する生物種: Orf virus (strain NZ2) (ORFV); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41213.77

構造登録者

Knapp, K.M.,Nakatani, Y.,Krause, K.L. (登録日: 2015-04-30, 公開日: 2015-07-08, 最終更新日: 2024-11-20)

主引用文献

Counago, R.M.,Knapp, K.M.,Nakatani, Y.,Fleming, S.B.,Corbett, M.,Wise, L.M.,Mercer, A.A.,Krause, K.L.
Structures of Orf Virus Chemokine Binding Protein in Complex with Host Chemokines Reveal Clues to Broad Binding Specificity.
Structure, 23:1199-1213, 2015

PubMed Abstract: The chemokine binding protein (CKBP) from orf virus (ORFV) binds with high affinity to chemokines from three classes, C, CC, and CXC, making it unique among poxvirus CKBPs described to date. We present its crystal structure alone and in complex with three CC chemokines, CCL2, CCL3, and CCL7. ORFV CKBP possesses a β-sandwich fold that is electrostatically and sterically complementary to its binding partners. Chemokines bind primarily through interactions involving the N-terminal loop and a hydrophobic recess on the ORFV CKBP β-sheet II surface, and largely polar interactions between the chemokine 20s loop and a negatively charged surface groove located at one end of the CKBP β-sheet II surface. ORFV CKBP interacts with leukocyte receptor and glycosaminoglycan binding sites found on the surface of bound chemokines. SEC-MALLS and chromatographic evidence is presented supporting that ORFV CKBP is a dimer in solution over a broad range of protein concentrations.

PubMed: 26095031
DOI: 10.1016/j.str.2015.04.023

実験手法

X-RAY DIFFRACTION (2.6 Å)