4ZFS

Phototoxic Fluorescent Protein KillerOrange

4ZFS の概要

• エントリーDOI: 10.2210/pdb4zfs/pdb
• 関連するPDBエントリー: 4ZBL
• 分子名称: KillerOrange (2 entities in total)
• 機能のキーワード: fluorescent protein, genetically encoded photosensitizer, chromophore-assisted light inactivation
• 由来する生物種: Hydrozoa (hydrozoans)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 138210.58

構造登録者

Pletneva, N.V.,Pletnev, V.Z.,Pletnev, S. (登録日: 2015-04-21, 公開日: 2015-12-23, 最終更新日: 2024-11-06)

主引用文献

Pletneva, N.V.,Pletnev, V.Z.,Sarkisyan, K.S.,Gorbachev, D.A.,Egorov, E.S.,Mishin, A.S.,Lukyanov, K.A.,Dauter, Z.,Pletnev, S.
Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.
Plos One, 10:e0145740-e0145740, 2015

PubMed Abstract: Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. Here, we present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKillerOrange, at 1.81 and 1.57 Å resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the β-barrel exterior and enabling transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers.

PubMed: 26699366
DOI: 10.1371/journal.pone.0145740

実験手法

X-RAY DIFFRACTION (2.01 Å)