4ZFP

A new crystal structure for the adduct formed in the reaction between AuSac2, a cytotoxic homoleptic gold(I) compound with the saccharinate ligand, and the model protein hen egg white lysozyme

4ZFP の概要

• エントリーDOI: 10.2210/pdb4zfp/pdb
• 分子名称: Lysozyme C, 1,2-ETHANEDIOL, NITRATE ION, ... (5 entities in total)
• 機能のキーワード: gold compounds, gold-based drugs, protein-metallodrug interactions, metallodrugs, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15356.16

構造登録者

Merlino, A. (登録日: 2015-04-21, 公開日: 2015-06-24, 最終更新日: 2024-11-20)

主引用文献

Ferraro, G.,Massai, L.,Messori, L.,Cinellu, M.A.,Merlino, A.
Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme.
Biometals, 28:745-754, 2015

PubMed Abstract: A new crystal structure is reported here for the adduct formed in the reaction between NH4 [Au(Sac)2], AuSac2, a cytotoxic homoleptic gold(I) complex with the saccharinate ligand, and the model protein hen egg white lysozyme. To produce this adduct, AuSac2 breaks down and releases both saccharinate ligands. The resulting Au(I) ions bind the protein to ND1 and NE2 atoms of His15 but also to SD atom of the zero-solvent accessible Met105 side chain, which is located in the protein hydrophobic box. The unexpected existence of this secondary gold(I) binding site is confirmed by spectroscopic and spectrometric measurements in solution.

PubMed: 26054833
DOI: 10.1007/s10534-015-9863-7

実験手法

X-RAY DIFFRACTION (1.96 Å)