4ZDV

Crystal structure of LC3 in complex with FAM134B LIR

Summary for 4ZDV

• Entry DOI: 10.2210/pdb4zdv/pdb
• Descriptor: Microtubule-associated proteins 1A/1B light chain 3A (2 entities in total)
• Functional Keywords: autophagy, er-phagy, complex, protein transport
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm, cytoskeleton: Q9H492
• Total number of polymer chains: 1
• Total formula weight: 15389.45

Authors

Khaminets, A.,Grumati, P.,Dikic, I.,Akutsu, M. (deposition date: 2015-04-19, release date: 2015-06-03, Last modification date: 2024-01-10)

Primary citation

Khaminets, A.,Heinrich, T.,Mari, M.,Grumati, P.,Huebner, A.K.,Akutsu, M.,Liebmann, L.,Stolz, A.,Nietzsche, S.,Koch, N.,Mauthe, M.,Katona, I.,Qualmann, B.,Weis, J.,Reggiori, F.,Kurth, I.,Hubner, C.A.,Dikic, I.
Regulation of endoplasmic reticulum turnover by selective autophagy.
Nature, 522:354-358, 2015

PubMed Abstract: The endoplasmic reticulum (ER) is the largest intracellular endomembrane system, enabling protein and lipid synthesis, ion homeostasis, quality control of newly synthesized proteins and organelle communication. Constant ER turnover and modulation is needed to meet different cellular requirements and autophagy has an important role in this process. However, its underlying regulatory mechanisms remain unexplained. Here we show that members of the FAM134 reticulon protein family are ER-resident receptors that bind to autophagy modifiers LC3 and GABARAP, and facilitate ER degradation by autophagy ('ER-phagy'). Downregulation of FAM134B protein in human cells causes an expansion of the ER, while FAM134B overexpression results in ER fragmentation and lysosomal degradation. Mutant FAM134B proteins that cause sensory neuropathy in humans are unable to act as ER-phagy receptors. Consistently, disruption of Fam134b in mice causes expansion of the ER, inhibits ER turnover, sensitizes cells to stress-induced apoptotic cell death and leads to degeneration of sensory neurons. Therefore, selective ER-phagy via FAM134 proteins is indispensable for mammalian cell homeostasis and controls ER morphology and turnover in mice and humans.

PubMed: 26040720
DOI: 10.1038/nature14498

Experimental method

X-RAY DIFFRACTION (1.8 Å)