4ZBZ
Family 4 uracil-DNA glycosylase from Sulfolobus tokodaii (free form, X-ray wavelength=1.5418)
Summary for 4ZBZ
| Entry DOI | 10.2210/pdb4zbz/pdb |
| Related | 4ZBX 4ZBY |
| Descriptor | Uracil-DNA glycosylase, IRON/SULFUR CLUSTER, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (5 entities in total) |
| Functional Keywords | uracil-dna glycosylase, dna repair, hydrolase |
| Biological source | Sulfolobus tokodaii str. 7 |
| Total number of polymer chains | 1 |
| Total formula weight | 22999.01 |
| Authors | Kawai, A.,Miyamoto, S. (deposition date: 2015-04-15, release date: 2015-09-09, Last modification date: 2023-11-08) |
| Primary citation | Kawai, A.,Higuchi, S.,Tsunoda, M.,Nakamura, K.T.,Yamagata, Y.,Miyamoto, S. Crystal structure of family 4 uracil-DNA glycosylase from Sulfolobus tokodaii and a function of tyrosine 170 in DNA binding Febs Lett., 589:2675-2682, 2015 Cited by PubMed Abstract: Uracil-DNA glycosylases (UDGs) excise uracil from DNA by catalyzing the N-glycosidic bond hydrolysis. Here we report the first crystal structures of an archaeal UDG (stoUDG). Compared with other UDGs, stoUDG has a different structure of the leucine-intercalation loop, which is important for DNA binding. The stoUDG-DNA complex model indicated that Leu169, Tyr170, and Asn171 in the loop are involved in DNA intercalation. Mutational analysis showed that Tyr170 is critical for substrate DNA recognition. These results indicate that Tyr170 occupies the intercalation site formed after the structural change of the leucine-intercalation loop required for the catalysis. PubMed: 26318717DOI: 10.1016/j.febslet.2015.08.019 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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