4ZB1

Crystal Structure of Blue Chromoprotein sgBP from Stichodactyla Gigantea

4ZB1 の概要

• エントリーDOI: 10.2210/pdb4zb1/pdb
• 分子名称: Blue chromoprotein, sgBP, 2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL (3 entities in total)
• 機能のキーワード: blue chromoprotein, luminescent protein
• 由来する生物種: Stichodactyla gigantea
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56660.35

構造登録者

Lee, C.C.,Ching, C.Y.,Tsai, H.J.,Wang, A.H.J. (登録日: 2015-04-14, 公開日: 2015-08-12, 最終更新日: 2026-03-18)

主引用文献

Chiang, C.Y.,Lee, C.C.,Lo, S.Y.,Wang, A.H.,Tsai, H.J.
Chromophore Deprotonation State Alters the Optical Properties of Blue Chromoprotein
Plos One, 10:e0134108-e0134108, 2015

PubMed Abstract: Chromoproteins (CPs) have unique colors and can be used in biological applications. In this work, a novel blue CP with a maximum absorption peak (λmax) at 608 nm was identified from the carpet anemone Stichodactyla gigantea (sgBP). In vivo expression of sgBP in zebrafish would change the appearance of the fishes to have a blue color, indicating the potential biomarker function. To enhance the color properties, the crystal structure of sgBP at 2.25 Å resolution was determined to allow structure-based protein engineering. Among the mutations conducted in the Gln-Tyr-Gly chromophore and chromophore environment, a S157C mutation shifted the λmax to 604 nm with an extinction coefficient (ε) of 58,029 M-1·cm-1 and darkened the blue color expression. The S157C mutation in the sgBP chromophore environment could affect the color expression by altering the deprotonation state of the phenolic group in the chromophore. Our results provide a structural basis for the blue color enhancement of the biomarker development.

PubMed: 26218063
DOI: 10.1371/journal.pone.0134108

実験手法

X-RAY DIFFRACTION (2.25 Å)