4Z7L

Crystal structure of Cas6b

4Z7L の概要

• エントリーDOI: 10.2210/pdb4z7l/pdb
• 関連するPDBエントリー: 4Z7K
• 分子名称: Cas6b, RNA (5'-R(*GP*CP*AP*AP*AP*AP*UP*AP*AP*CP*AP*AP*GP*C)-3'), SULFATE ION (3 entities in total)
• 機能のキーワード: crispr rna, endoribonucleases, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Methanococcus maripaludis (strain C5 / ATCC BAA-1333); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 167123.91

構造登録者

Li, H. (登録日: 2015-04-07, 公開日: 2016-04-13, 最終更新日: 2024-03-06)

主引用文献

Shao, Y.,Richter, H.,Sun, S.,Sharma, K.,Urlaub, H.,Randau, L.,Li, H.
A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.
Structure, 24:547-554, 2016

PubMed Abstract: A subclass of recently discovered CRISPR repeat RNA in bacteria contains minimally recognizable structural features that facilitate an unknown mechanism of recognition and processing by the Cas6 family of endoribonucleases. Cocrystal structures of Cas6 from Methanococcus maripaludis (MmCas6b) bound with its repeat RNA revealed a dual site binding structure and a cleavage site conformation poised for phosphodiester bond breakage. Two non-interacting MmCas6b bind to two separate AAYAA motifs within the same repeat, one distal and one adjacent to the cleavage site. This bound structure potentially competes with a stable but non-productive RNA structure. At the cleavage site, MmCas6b supplies a base pair mimic to stabilize a short 2 base pair stem immediately upstream of the scissile phosphate. Complementary biochemical analyses support the dual-AAYAA binding model and a critical role of the protein-RNA base pair mimic. Our results reveal a previously unknown method of processing non-stem-loop CRISPR RNA by Cas6.

PubMed: 26996962
DOI: 10.1016/j.str.2016.02.009

実験手法

X-RAY DIFFRACTION (3.503 Å)