4Z43
Crystal structure of Tryptophan 7-halogenase (PrnA) Mutant E450K
Summary for 4Z43
| Entry DOI | 10.2210/pdb4z43/pdb |
| Descriptor | Flavin-dependent tryptophan halogenase PrnA, FLAVIN-ADENINE DINUCLEOTIDE, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | oxidoreductase, 7-halogenase (prna) mutant e450k |
| Biological source | Pseudomonas fluorescens |
| Total number of polymer chains | 1 |
| Total formula weight | 62063.22 |
| Authors | Levy, C.W. (deposition date: 2015-04-01, release date: 2016-01-20, Last modification date: 2024-01-10) |
| Primary citation | Shepherd, S.A.,Karthikeyan, C.,Latham, J.,Struck, A.W.,Thompson, M.L.,Menon, B.R.K.,Styles, M.Q.,Levy, C.,Leys, D.,Micklefield, J. Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes. Chem Sci, 6:3454-3460, 2015 Cited by PubMed Abstract: Flavin-dependent halogenases are potentially valuable biocatalysts for the regioselective halogenation of aromatic compounds. These enzymes, utilising benign inorganic halides, offer potential advantages over traditional non-enzymatic halogenation chemistry that often lacks regiocontrol and requires deleterious reagents. Here we extend the biocatalytic repertoire of the tryptophan halogenases, demonstrating how these enzymes can halogenate a range of alternative aryl substrates. Using structure guided mutagenesis we also show that it is possible to alter the regioselectivity as well as increase the activity of the halogenases with non-native substrates including anthranilic acid; an important intermediate in the synthesis and biosynthesis of pharmaceuticals and other valuable products. PubMed: 29511510DOI: 10.1039/c5sc00913h PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.29 Å) |
Structure validation
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