4Z1A

Structure of apo form KDO8PS from H.pylori

4Z1A の概要

• エントリーDOI: 10.2210/pdb4z1a/pdb
• 関連するPDBエントリー: 4Z1B 4Z1C 4Z1D
• 分子名称: 2-dehydro-3-deoxyphosphooctonate aldolase (2 entities in total)
• 機能のキーワード: helicobacter pylori, lipopolysaccharide biosynthesis, apo, transferase
• 由来する生物種: Helicobacter pylori (strain ATCC 700392 / 26695)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60763.83

構造登録者

Lee, B.J.,Cho, S.,Im, H.,Yoon, H.J. (登録日: 2015-03-27, 公開日: 2016-03-09, 最終更新日: 2024-03-20)

主引用文献

Cho, S.,Im, H.,Lee, K.Y.,Chen, J.,Kang, H.J.,Yoon, H.J.,Min, K.H.,Lee, K.R.,Park, H.J.,Lee, B.J.
Identification of novel scaffolds for potential anti-Helicobacter pylori agents based on the crystal structure of H. pylori 3-deoxy-d-manno-octulosonate 8-phosphate synthase (HpKDO8PS).
Eur.J.Med.Chem., 108:188-202, 2016

PubMed Abstract: The crystal structure of 3-deoxy-d-manno-octulosonate-8-phosphate synthase (KDO8PS) from Helicobacter pylori (HpKDO8PS) was determined alone and within various complexes, revealing an extra helix (HE) that is absent in the structures of KDO8PS from other organisms. In contrast to the metal coordination of the KDO8PS enzyme from Aquifex aeolicus, HpKDO8PS is specifically coordinated with Cd(2+) or Zn(2+) ions, and isothermal titration calorimetry (ITC) and differential scanning fluorimetry (DSF) revealed that Cd(2+) thermally stabilizes the protein structure more efficiently than Zn(2+). In the substrate-bound structure, water molecules play a key role in fixing residues in the proper configuration to achieve a compact structure. Using the structures of HpKDO8PS and API [arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) bisubstrate inhibitor], we generated 21 compounds showing potential HpKDO8PS-binding properties via in silico virtual screening. The capacity of three, avicularin, hyperin, and MC181, to bind to HpKDO8PS was confirmed through saturation transfer difference (STD) experiments, and we identified their specific ligand binding modes by combining competition experiments and docking simulation analysis. Hyperin was confirmed to bind to the A5P binding site, primarily via hydrophilic interaction, whereas MC181 bound to both the PEP and A5P binding sites through hydrophilic and hydrophobic interactions. These results were consistent with the epitope mapping by STD. Our results are expected to provide clues for the development of HpKDO8PS inhibitors.

PubMed: 26649906
DOI: 10.1016/j.ejmech.2015.11.036

実験手法

X-RAY DIFFRACTION (2 Å)