4YX7

Complex of SpaO(SPOA1,2) and OrgB(APAR)::T4lysozyme fusion protein

4YX7 の概要

• エントリーDOI: 10.2210/pdb4yx7/pdb
• 分子名称: Surface presentation of antigens protein SpaO, Oxygen-regulated invasion protein OrgB,Endolysin, ... (4 entities in total)
• 機能のキーワード: type iii secretion system, protein transport
• 由来する生物種: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720); 詳細
• 細胞内の位置: Host cytoplasm : P00720
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 76239.90

構造登録者

Notti, R.Q.,Stebbins, C.E. (登録日: 2015-03-22, 公開日: 2015-06-03, 最終更新日: 2023-09-27)

主引用文献

Notti, R.Q.,Bhattacharya, S.,Lilic, M.,Stebbins, C.E.
A common assembly module in injectisome and flagellar type III secretion sorting platforms.
Nat Commun, 6:7125-7125, 2015

PubMed Abstract: Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.

PubMed: 25994170
DOI: 10.1038/ncomms8125

実験手法

X-RAY DIFFRACTION (2.0007 Å)