4YWL
Pyrococcus furiosus MCM N-terminal domain F179A point mutant pentameric ring
4YWL の概要
エントリーDOI | 10.2210/pdb4ywl/pdb |
関連するPDBエントリー | 4POF 4YWK 4YWM |
分子名称 | Cell division control protein 21, ZINC ION, SULFATE ION (3 entities in total) |
機能のキーワード | mcm, helicase, replication, ob-fold, cell cycle |
由来する生物種 | Pyrococcus furiosus |
タンパク質・核酸の鎖数 | 10 |
化学式量合計 | 294764.83 |
構造登録者 | |
主引用文献 | Froelich, C.A.,Nourse, A.,Enemark, E.J. MCM ring hexamerization is a prerequisite for DNA-binding. Nucleic Acids Res., 43:9553-9563, 2015 Cited by PubMed Abstract: The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings by showing that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in the hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association. PubMed: 26365238DOI: 10.1093/nar/gkv914 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (3.2 Å) |
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