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4YWL

Pyrococcus furiosus MCM N-terminal domain F179A point mutant pentameric ring

4YWL の概要
エントリーDOI10.2210/pdb4ywl/pdb
関連するPDBエントリー4POF 4YWK 4YWM
分子名称Cell division control protein 21, ZINC ION, SULFATE ION (3 entities in total)
機能のキーワードmcm, helicase, replication, ob-fold, cell cycle
由来する生物種Pyrococcus furiosus
タンパク質・核酸の鎖数10
化学式量合計294764.83
構造登録者
Froelich, C.A.,Enemark, E.J. (登録日: 2015-03-20, 公開日: 2015-09-23, 最終更新日: 2023-09-27)
主引用文献Froelich, C.A.,Nourse, A.,Enemark, E.J.
MCM ring hexamerization is a prerequisite for DNA-binding.
Nucleic Acids Res., 43:9553-9563, 2015
Cited by
PubMed Abstract: The hexameric Minichromosome Maintenance (MCM) protein complex forms a ring that unwinds DNA at the replication fork in eukaryotes and archaea. Our recent crystal structure of an archaeal MCM N-terminal domain bound to single-stranded DNA (ssDNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indicating that DNA-binding is governed not only by the DNA-binding residues of the subunits (MCM ssDNA-binding motif, MSSB) but also by the relative orientation of the subunits. We now extend these findings by showing that DNA-binding by the MCM N-terminal domain of the archaeal organism Pyrococcus furiosus occurs specifically in the hexameric oligomeric form. We show that mutants defective for hexamerization are defective in binding ssDNA despite retaining all the residues observed to interact with ssDNA in the crystal structure. One mutation that exhibits severely defective hexamerization and ssDNA-binding is at a conserved phenylalanine that aligns with the mouse Mcm4(Chaos3) mutation associated with chromosomal instability, cancer, and decreased intersubunit association.
PubMed: 26365238
DOI: 10.1093/nar/gkv914
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (3.2 Å)
構造検証レポート
Validation report summary of 4ywl
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-06に公開中

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