4YRV

Crystal structure of Anabaena transcription factor HetR complexed with 21-bp DNA from hetP promoter

「4K1M」から置き換えられました

4YRV の概要

• エントリーDOI: 10.2210/pdb4yrv/pdb
• 関連するPDBエントリー: 3QOD 4HRI
• 分子名称: Heterocyst differentiation control protein, DNA (5'-D(P*GP*CP*GP*AP*GP*GP*GP*GP*TP*CP*TP*AP*AP*CP*CP*CP*CP*TP*CP*AP*T)-3'), DNA (5'-D(P*AP*TP*GP*AP*GP*GP*GP*GP*TP*TP*AP*GP*AP*CP*CP*CP*CP*TP*CP*GP*C)-3'), ... (5 entities in total)
• 機能のキーワード: heterocyst differentiation, transcription factor, complex, transcription-dna complex, transcription/dna
• 由来する生物種: Nostoc sp. PCC 7120; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 85117.52

構造登録者

Hu, H.X.,Jiang, Y.L.,Zhao, M.X.,Zhang, C.C.,Chen, Y.,Zhou, C.Z. (登録日: 2015-03-16, 公開日: 2015-12-02, 最終更新日: 2023-11-08)

主引用文献

Hu, H.X.,Jiang, Y.L.,Zhao, M.X.,Cai, K.,Liu, S.,Wen, B.,Lv, P.,Zhang, Y.,Peng, J.,Zhong, H.,Yu, H.M.,Ren, Y.M.,Zhang, Z.,Tian, C.,Wu, Q.,Oliveberg, M.,Zhang, C.C.,Chen, Y.,Zhou, C.Z.
Structural insights into HetR-PatS interaction involved in cyanobacterial pattern formation
Sci Rep, 5:16470-16470, 2015

PubMed Abstract: The one-dimensional pattern of heterocyst in the model cyanobacterium Anabaena sp. PCC 7120 is coordinated by the transcription factor HetR and PatS peptide. Here we report the complex structures of HetR binding to DNA, and its hood domain (HetRHood) binding to a PatS-derived hexapeptide (PatS6) at 2.80 and 2.10 Å, respectively. The intertwined HetR dimer possesses a couple of novel HTH motifs, each of which consists of two canonical α-helices in the DNA-binding domain and an auxiliary α-helix from the flap domain of the neighboring subunit. Two PatS6 peptides bind to the lateral clefts of HetRHood, and trigger significant conformational changes of the flap domain, resulting in dissociation of the auxiliary α-helix and eventually release of HetR from the DNA major grove. These findings provide the structural insights into a prokaryotic example of Turing model.

PubMed: 26576507
DOI: 10.1038/srep16470

実験手法

X-RAY DIFFRACTION (2.8 Å)