4YO2

Structure of E2F8, an atypical member of E2F family of transcription factors

4YO2 の概要

• エントリーDOI: 10.2210/pdb4yo2/pdb
• 分子名称: Transcription factor E2F8, DNA (5'-D(P*TP*TP*TP*TP*CP*CP*CP*GP*CP*CP*AP*AP*AP*AP*A)-3'), DNA (5'-D(P*TP*TP*TP*TP*TP*GP*GP*CP*GP*GP*GP*AP*AP*AP*A)-3') (3 entities in total)
• 機能のキーワード: transcription factor, e2f family, dna-complex, transcription, fusion protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : A0AVK6
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 36080.12

構造登録者

Morgunova, E.,Yin, Y.,Jolma, A.,Dave, K.,Schmierer, B.,Popov, A.,Eremina, N.,Nilsson, L.,Taipale, J. (登録日: 2015-03-11, 公開日: 2015-12-09, 最終更新日: 2024-01-10)

主引用文献

Morgunova, E.,Yin, Y.,Jolma, A.,Dave, K.,Schmierer, B.,Popov, A.,Eremina, N.,Nilsson, L.,Taipale, J.
Structural insights into the DNA-binding specificity of E2F family transcription factors.
Nat Commun, 6:10050-10050, 2015

PubMed Abstract: The mammalian cell cycle is controlled by the E2F family of transcription factors. Typical E2Fs bind to DNA as heterodimers with the related dimerization partner (DP) proteins, whereas the atypical E2Fs, E2F7 and E2F8 contain two DNA-binding domains (DBDs) and act as repressors. To understand the mechanism of repression, we have resolved the structure of E2F8 in complex with DNA at atomic resolution. We find that the first and second DBDs of E2F8 resemble the DBDs of typical E2F and DP proteins, respectively. Using molecular dynamics simulations, biochemical affinity measurements and chromatin immunoprecipitation, we further show that both atypical and typical E2Fs bind to similar DNA sequences in vitro and in vivo. Our results represent the first crystal structure of an E2F protein with two DBDs, and reveal the mechanism by which atypical E2Fs can repress canonical E2F target genes and exert their negative influence on cell cycle progression.

PubMed: 26632596
DOI: 10.1038/ncomms10050

実験手法

X-RAY DIFFRACTION (3.073 Å)