4YH8

Structure of yeast U2AF complex

4YH8 の概要

• エントリーDOI: 10.2210/pdb4yh8/pdb
• 分子名称: Splicing factor U2AF 23 kDa subunit, Splicing factor U2AF 59 kDa subunit, ZINC ION, ... (4 entities in total)
• 機能のキーワード: splicing, rrm, zn-finger, 3' splice site
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33140.05

構造登録者

Yoshida, H.,Park, S.Y.,Urano, T.,Obayashi, E. (登録日: 2015-02-27, 公開日: 2015-08-12, 最終更新日: 2024-03-20)

主引用文献

Yoshida, H.,Park, S.Y.,Oda, T.,Akiyoshi, T.,Sato, M.,Shirouzu, M.,Tsuda, K.,Kuwasako, K.,Unzai, S.,Muto, Y.,Urano, T.,Obayashi, E.
A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit.
Genes Dev., 29:1649-1660, 2015

PubMed Abstract: The pre-mRNA splicing reaction of eukaryotic cells has to be carried out extremely accurately, as failure to recognize the splice sites correctly causes serious disease. The small subunit of the U2AF heterodimer is essential for the determination of 3' splice sites in pre-mRNA splicing, and several single-residue mutations of the U2AF small subunit cause severe disorders such as myelodysplastic syndromes. However, the mechanism of RNA recognition is poorly understood. Here we solved the crystal structure of the U2AF small subunit (U2AF23) from fission yeast, consisting of an RNA recognition motif (RRM) domain flanked by two conserved CCCH-type zinc fingers (ZFs). The two ZFs are positioned side by side on the β sheet of the RRM domain. Further mutational analysis revealed that the ZFs bind cooperatively to the target RNA sequence, but the RRM domain acts simply as a scaffold to organize the ZFs and does not itself contact the RNA directly. This completely novel and unexpected mode of RNA-binding mechanism by the U2AF small subunit sheds light on splicing errors caused by mutations of this highly conserved protein.

PubMed: 26215567
DOI: 10.1101/gad.267104.115

実験手法

X-RAY DIFFRACTION (1.7 Å)