4YH3

Crystal structure of human BRD4(1) in complex with 4-[(2E)-3-(4-methoxyphenyl)-2-phenylprop-2-enoyl]-3,4-dihydroquinoxalin-2(1H)-one (compound 19a)

Summary for 4YH3

• Entry DOI: 10.2210/pdb4yh3/pdb
• Related: 4YH4
• Descriptor: Bromodomain-containing protein 4, 4-[(2E)-3-(4-methoxyphenyl)-2-phenylprop-2-enoyl]-3,4-dihydroquinoxalin-2(1H)-one (3 entities in total)
• Functional Keywords: brd4(1), protein binding
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus: O60885
• Total number of polymer chains: 1
• Total formula weight: 15714.02

Authors

White, A.,Lakshminarasimhan, D.,Suto, R.K. (deposition date: 2015-02-26, release date: 2016-01-13, Last modification date: 2024-02-28)

Primary citation

Duffy, B.C.,Liu, S.,Martin, G.S.,Wang, R.,Hsia, M.M.,Zhao, H.,Guo, C.,Ellis, M.,Quinn, J.F.,Kharenko, O.A.,Norek, K.,Gesner, E.M.,Young, P.R.,McLure, K.G.,Wagner, G.S.,Lakshminarasimhan, D.,White, A.,Suto, R.K.,Hansen, H.C.,Kitchen, D.B.
Discovery of a new chemical series of BRD4(1) inhibitors using protein-ligand docking and structure-guided design.
Bioorg.Med.Chem.Lett., 25:2818-2823, 2015

PubMed Abstract: Bromodomains are key transcriptional regulators that are thought to be druggable epigenetic targets for cancer, inflammation, diabetes and cardiovascular therapeutics. Of particular importance is the first of two bromodomains in bromodomain containing 4 protein (BRD4(1)). Protein-ligand docking in BRD4(1) was used to purchase a small, focused screening set of compounds possessing a large variety of core structures. Within this set, a small number of weak hits each contained a dihydroquinoxalinone ring system. We purchased other analogs with this ring system and further validated the new hit series and obtained improvement in binding inhibition. Limited exploration by new analog synthesis showed that the binding inhibition in a FRET assay could be improved to the low μM level making this new core a potential hit-to-lead series. Additionally, the predicted geometries of the initial hit and an improved analog were confirmed by X-ray co-crystallography with BRD4(1).

PubMed: 26022843
DOI: 10.1016/j.bmcl.2015.04.107

Experimental method

X-RAY DIFFRACTION (1.6 Å)