4YCZ

Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA)

4YCZ の概要

• エントリーDOI: 10.2210/pdb4ycz/pdb
• 分子名称: Fusion Protein of Sec13 and Nup145C, Nup85, Nup120 (3 entities in total)
• 機能のキーワード: structural protein complex, nuclear pore complex, macromolecular assemblies, structural protein
• 由来する生物種: Thielavia heterothallica; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 234483.05

構造登録者

Kelley, K.,Knockenhauer, K.E.,Schwartz, T.U. (登録日: 2015-02-20, 公開日: 2015-04-01, 最終更新日: 2024-10-23)

主引用文献

Kelley, K.,Knockenhauer, K.E.,Kabachinski, G.,Schwartz, T.U.
Atomic structure of the Y complex of the nuclear pore.
Nat.Struct.Mol.Biol., 22:425-431, 2015

PubMed Abstract: The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. Here we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.

PubMed: 25822992
DOI: 10.1038/nsmb.2998

実験手法

X-RAY DIFFRACTION (4.1 Å)