4Y7E

Crystal structure of beta-mannanase from Streptomyces thermolilacinus with mannohexaose

4Y7E の概要

• エントリーDOI: 10.2210/pdb4y7e/pdb
• 関連するPDBエントリー: 3WSU
• 分子名称: Endoglucanase, beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose, beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose, ... (7 entities in total)
• 機能のキーワード: mannanase, glycoside hydrolase family 5, actinomycete, hydrolase
• 由来する生物種: Streptomyces thermolilacinus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78438.38

構造登録者

Kumagai, Y.,Yamashita, K.,Okuyama, M.,Hatanaka, T.,Yao, M.,Kimura, A. (登録日: 2015-02-14, 公開日: 2015-09-23, 最終更新日: 2023-11-08)

主引用文献

Kumagai, Y.,Yamashita, K.,Tagami, T.,Uraji, M.,Wan, K.,Okuyama, M.,Yao, M.,Kimura, A.,Hatanaka, T.
The loop structure of Actinomycete glycoside hydrolase family 5 mannanases governs substrate recognition
Febs J., 282:4001-4014, 2015

PubMed Abstract: Endo-β-1,4-mannanases from Streptomyces thermolilacinus (StMan) and Thermobifida fusca (TfMan) demonstrated different substrate specificities. StMan hydrolyzed galactosylmannooligosaccharide (GGM5; 6(III) ,6(IV) -α-d-galactosyl mannopentaose) to GGM3 and M2, whereas TfMan hydrolyzed GGM5 to GGM4 and M1. To determine the region involved in the substrate specificity, we constructed chimeric enzymes of StMan and TfMan and evaluated their substrate specificities. Moreover, the crystal structure of the catalytic domain of StMan (StMandC) and the complex structure of the inactive mutant StE273AdC with M6 were solved at resolutions of 1.60 and 1.50 Å, respectively. Structural comparisons of StMandC and the catalytic domain of TfMan lead to the identification of a subsite around -1 in StMandC that could accommodate a galactose branch. These findings demonstrate that the two loops (loop7 and loop8) are responsible for substrate recognition in GH5 actinomycete mannanases. In particular, Trp281 in loop7 of StMan, which is located in a narrow and deep cleft, plays an important role in its affinity toward linear substrates. Asp310 in loop8 of StMan specifically bound to the galactosyl unit in the -1 subsite.

PubMed: 26257335
DOI: 10.1111/febs.13401

実験手法

X-RAY DIFFRACTION (1.5 Å)