4Y5F
PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, high dose
Summary for 4Y5F
| Entry DOI | 10.2210/pdb4y5f/pdb |
| Related | 4Y3I |
| Descriptor | Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid (3 entities in total) |
| Functional Keywords | high-dose, spectroscopy, bilin chromophore, phythochrome, transferase |
| Biological source | Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) |
| Total number of polymer chains | 1 |
| Total formula weight | 35048.99 |
| Authors | Li, F.,Burgie, E.S.,Yu, T.,Heroux, A.,Schatz, G.C.,Vierstra, R.D.,Orville, A.M. (deposition date: 2015-02-11, release date: 2015-05-20, Last modification date: 2024-11-06) |
| Primary citation | Li, F.,Burgie, E.S.,Yu, T.,Heroux, A.,Schatz, G.C.,Vierstra, R.D.,Orville, A.M. X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. radiodurans phytochrome. J.Am.Chem.Soc., 137:2792-2795, 2015 Cited by PubMed Abstract: We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins. PubMed: 25650486DOI: 10.1021/ja510923m PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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