4Y5F

PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, high dose

4Y5F の概要

• エントリーDOI: 10.2210/pdb4y5f/pdb
• 関連するPDBエントリー: 4Y3I
• 分子名称: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid (3 entities in total)
• 機能のキーワード: high-dose, spectroscopy, bilin chromophore, phythochrome, transferase
• 由来する生物種: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35048.99

構造登録者

Li, F.,Burgie, E.S.,Yu, T.,Heroux, A.,Schatz, G.C.,Vierstra, R.D.,Orville, A.M. (登録日: 2015-02-11, 公開日: 2015-05-20, 最終更新日: 2024-11-06)

主引用文献

Li, F.,Burgie, E.S.,Yu, T.,Heroux, A.,Schatz, G.C.,Vierstra, R.D.,Orville, A.M.
X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. radiodurans phytochrome.
J.Am.Chem.Soc., 137:2792-2795, 2015

PubMed Abstract: We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins.

PubMed: 25650486
DOI: 10.1021/ja510923m

実験手法

X-RAY DIFFRACTION (1.7 Å)