4Y5D

CRYSTAL STRUCTURE OF ALiS2-STREPTAVIDIN COMPLEX

4Y5D の概要

• エントリーDOI: 10.2210/pdb4y5d/pdb
• 関連するPDBエントリー: 4Y59
• 分子名称: Streptavidin, methyl 3-(4-oxo-4,5-dihydrofuro[3,2-c]pyridin-2-yl)benzoate, 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL, ... (6 entities in total)
• 機能のキーワード: biotin-binding protein, inhibitor
• 由来する生物種: Streptomyces avidinii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53492.95

構造登録者

Sugiyama, S.,Terai, T.,Kohno, M.,Ishida, H.,Nagano, T. (登録日: 2015-02-11, 公開日: 2015-09-23, 最終更新日: 2024-03-20)

主引用文献

Terai, T.,Kohno, M.,Boncompain, G.,Sugiyama, S.,Saito, N.,Fujikake, R.,Ueno, T.,Komatsu, T.,Hanaoka, K.,Okabe, T.,Urano, Y.,Perez, F.,Nagano, T.
Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport
J.Am.Chem.Soc., 137:10464-10467, 2015

PubMed Abstract: Artificial ligands of streptavidin (ALiS) with association constants of ∼10(6) M(-1) were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.

PubMed: 26261872
DOI: 10.1021/jacs.5b05672

実験手法

X-RAY DIFFRACTION (1.2 Å)