4Y4O

Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to protein Y (YfiA) at 2.3A resolution

This is a large structure.

Summary for 4Y4O

Related4Y4P
Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (59 entities in total)
Functional Keywordsribosome, rrna modofications, pseudouridine, rrna methylation, ribosomal protein post-translational modifications, iron-sulfur cluster, beta-thio-aspartate
Biological sourceEscherichia coli (strain K12)
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Total number of polymer chains106
Total molecular weight4426437.56
Authors
Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A. (deposition date: 2015-02-10, release date: 2015-03-18, Last modification date: 2017-09-27)
Primary citation
Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A.
Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.
Nat.Struct.Mol.Biol., 22:342-344, 2015
PubMed: 25775268 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.2992
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.24780.5%9.3%6.8%0.56MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4y4o
no rotation
Molmil generated image of 4y4o
rotated about x axis by 90°
Molmil generated image of 4y4o
rotated about y axis by 90°