Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to protein Y (YfiA) at 2.3A resolution

This is a large structure.

Summary for 4Y4O

Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (59 entities in total)
Functional Keywordsribosome, rrna modofications, pseudouridine, rrna methylation, ribosomal protein post-translational modifications, iron-sulfur cluster, beta-thio-aspartate
Biological sourceEscherichia coli (strain K12)
Total number of polymer chains106
Total molecular weight4426437.56
Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A. (deposition date: 2015-02-10, release date: 2015-03-18, Last modification date: 2017-09-27)
Primary citation
Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A.
Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.
Nat.Struct.Mol.Biol., 22:342-344, 2015
PubMed: 25775268 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.2992
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.24780.5%9.3%6.8%0.56MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4y4o
no rotation
Molmil generated image of 4y4o
rotated about x axis by 90°
Molmil generated image of 4y4o
rotated about y axis by 90°