4Y4O

Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to protein Y (YfiA) at 2.3A resolution

This is a non-PDB format compatible entry.

Summary for 4Y4O

• Entry DOI: 10.2210/pdb4y4o/pdb
• Related: 4Y4P
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (59 entities in total)
• Functional Keywords: ribosome, rrna modofications, pseudouridine, rrna methylation, ribosomal protein post-translational modifications, iron-sulfur cluster, beta-thio-aspartate
• Biological source: Escherichia coli (strain K12); More
• Total number of polymer chains: 106
• Total formula weight: 4426437.55

Authors

Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A. (deposition date: 2015-02-10, release date: 2015-03-18, Last modification date: 2023-11-15)

Primary citation

Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A.
Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.
Nat.Struct.Mol.Biol., 22:342-344, 2015

PubMed Abstract: We report crystal structures of the Thermus thermophilus ribosome at 2.3- to 2.5-Å resolution, which have enabled modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields a more comprehensive atomic model of a bacterial ribosome.

PubMed: 25775268
DOI: 10.1038/nsmb.2992

Experimental method

X-RAY DIFFRACTION (2.3 Å)