4XZC

The crystal structure of Kupe virus nucleoprotein

4XZC の概要

• エントリーDOI: 10.2210/pdb4xzc/pdb
• 関連するPDBエントリー: 4XZ8 4XZC 4XZE
• 分子名称: Nucleoprotein (2 entities in total)
• 機能のキーワード: nucleoprotein, kupe virus, rna binding protein
• 由来する生物種: Kupe virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 108377.76

構造登録者

Guo, Y.,Wang, W.,Liu, X.,Wang, X.,Wang, J.,Huo, T.,Liu, B. (登録日: 2015-02-04, 公開日: 2015-09-02, 最終更新日: 2023-11-08)

主引用文献

Wang, W.,Liu, X.,Wang, X.,Dong, H.,Ma, C.,Wang, J.,Liu, B.,Mao, Y.,Wang, Y.,Li, T.,Yang, C.,Guo, Y.
Structural and Functional Diversity of Nairovirus-Encoded Nucleoproteins.
J.Virol., 89:11740-11749, 2015

PubMed Abstract: The nairoviruses include assorted tick-borne bunyaviruses that are emerging as causative agents of infectious diseases among humans and animals. As negative-sense single-stranded RNA (-ssRNA) viruses, nairoviruses encode nucleoprotein (NP) that encapsidates the genomic RNA and further forms ribonucleoprotein (RNP) complex with viral RNA-dependent RNA polymerase (RdRp). We previously revealed that the monomeric NP encoded by Crimean-Congo hemorrhagic fever virus (CCHFV) presents a racket-shaped structure and shows unusual DNA-specific endonuclease activity. To examine the structural and biological variation of nairovirus-encoded NPs, here, we systematically solved the crystal structures of NPs encoded by various nairoviruses, including Hazara virus (HAZV), Kupe virus (KUPV), and Erve virus (ERVEV). Combined with biochemical analysis, our results generate a clearer picture to aid in the understanding of the functional diversity of nairovirus-encoded NPs and the formation of nairovirus RNPs.

PubMed: 26246561
DOI: 10.1128/JVI.01680-15

実験手法

X-RAY DIFFRACTION (2.601 Å)