4XYD

Nitric oxide reductase from Roseobacter denitrificans (RdNOR)

4XYD の概要

• エントリーDOI: 10.2210/pdb4xyd/pdb
• 分子名称: Nitric-oxide reductase subunit B, NorC-like protein, PROTOPORPHYRIN IX CONTAINING FE, ... (9 entities in total)
• 機能のキーワード: nitric oxide reductase, membrane protein, heme copper oxidase superfamily, oxidoreductase
• 由来する生物種: Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70372.32

構造登録者

Crow, A. (登録日: 2015-02-02, 公開日: 2016-05-25, 最終更新日: 2024-11-06)

主引用文献

Crow, A.,Matsuda, Y.,Arata, H.,Oubrie, A.
Structure of the Membrane-intrinsic Nitric Oxide Reductase from Roseobacter denitrificans.
Biochemistry, 55:3198-3203, 2016

PubMed Abstract: Membrane-intrinsic nitric oxide reductases (NORs) are key components of bacterial denitrification pathways with a close evolutionary relationship to the cytochrome oxidase (COX) complex found in aerobic respiratory chains. A key distinction between COX and NOR is the identity of the metal directly opposite heme b3 within the active site. In NOR, this metal is iron (FeB), whereas in COX, it is copper (CuB). The purified NOR of Roseobacter denitrificans contains copper and has modest oxidase activity, raising the possibility that a COX-like active site might have independently arisen within the context of a NOR-like protein scaffold. Here we present the crystal structure of the Roseobacter denitrificans NorBC complex and anomalous scattering experiments probing the identity of each metal center. Our results refute the hypothesis that copper occupies the active site and instead reveal a new metal center in the small subunit not seen in any other NOR or COX.

PubMed: 27185533
DOI: 10.1021/acs.biochem.6b00332

実験手法

X-RAY DIFFRACTION (2.85 Å)