4XU5

Crystal structure of MvINS bound to a bromine-derived 14C Diacylglycerol (DAG) at 2.1A resolution

4XU5 の概要

• エントリーDOI: 10.2210/pdb4xu5/pdb
• 関連するPDBエントリー: 4XU4 4XU6 4XU7 4XU8 4XU9
• 分子名称: Uncharacterized protein, (2S)-1-[(13-bromotridecanoyl)oxy]-3-hydroxypropan-2-yl tetradecanoate, DECANE, ... (5 entities in total)
• 機能のキーワード: unknown function
• 由来する生物種: Mycobacterium vanbaalenii PYR-1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23134.51

構造登録者

Ren, R.B.,Wu, J.P.,Yan, C.Y.,He, Y.,Yan, N. (登録日: 2015-01-25, 公開日: 2015-10-14, 最終更新日: 2024-03-20)

主引用文献

Ren, R.,Zhou, X.,He, Y.,Ke, M.,Wu, J.,Liu, X.,Yan, C.,Wu, Y.,Gong, X.,Lei, X.,Yan, S.F.,Radhakrishnan, A.,Yan, N.
PROTEIN STRUCTURE. Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels
Science, 349:187-191, 2015

PubMed Abstract: Insulin-induced gene 1 (Insig-1) and Insig-2 are endoplasmic reticulum membrane-embedded sterol sensors that regulate the cellular accumulation of sterols. Despite their physiological importance, the structural information on Insigs remains limited. Here we report the high-resolution structures of MvINS, an Insig homolog from Mycobacterium vanbaalenii. MvINS exists as a homotrimer. Each protomer comprises six transmembrane segments (TMs), with TM3 and TM4 contributing to homotrimerization. The six TMs enclose a V-shaped cavity that can accommodate a diacylglycerol molecule. A homology-based structural model of human Insig-2, together with biochemical characterizations, suggest that the central cavity of Insig-2 accommodates 25-hydroxycholesterol, whereas TM3 and TM4 engage in Scap binding. These analyses provide an important framework for further functional and mechanistic understanding of Insig proteins and the sterol regulatory element-binding protein pathway.

PubMed: 26160948
DOI: 10.1126/science.aab1091

実験手法

X-RAY DIFFRACTION (2.1 Å)