4XT1

Structure of a nanobody-bound viral GPCR bound to human chemokine CX3CL1

4XT1 の概要

• エントリーDOI: 10.2210/pdb4xt1/pdb
• 関連するPDBエントリー: 4XT3
• 分子名称: G-protein coupled receptor homolog US28, Fractalkine, nanobody 7, ... (8 entities in total)
• 機能のキーワード: gpcr, chemokine, membrane protein, complex, viral protein-signalling protein complex, viral protein/signalling protein
• 由来する生物種: Cytomegalovirus; 詳細
• 細胞内の位置: Host cell membrane; Multi-pass membrane protein: P69332; Cell membrane; Single-pass type I membrane protein. Processed fractalkine: Secreted: P78423
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 69666.35

構造登録者

Burg, J.S.,Jude, K.M.,Waghray, D.,Garcia, K.C. (登録日: 2015-01-22, 公開日: 2015-03-04, 最終更新日: 2024-11-20)

主引用文献

Burg, J.S.,Ingram, J.R.,Venkatakrishnan, A.J.,Jude, K.M.,Dukkipati, A.,Feinberg, E.N.,Angelini, A.,Waghray, D.,Dror, R.O.,Ploegh, H.L.,Garcia, K.C.
Structural biology. Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor.
Science, 347:1113-1117, 2015

PubMed Abstract: Chemokines are small proteins that function as immune modulators through activation of chemokine G protein-coupled receptors (GPCRs). Several viruses also encode chemokines and chemokine receptors to subvert the host immune response. How protein ligands activate GPCRs remains unknown. We report the crystal structure at 2.9 angstrom resolution of the human cytomegalovirus GPCR US28 in complex with the chemokine domain of human CX3CL1 (fractalkine). The globular body of CX3CL1 is perched on top of the US28 extracellular vestibule, whereas its amino terminus projects into the central core of US28. The transmembrane helices of US28 adopt an active-state-like conformation. Atomic-level simulations suggest that the agonist-independent activity of US28 may be due to an amino acid network evolved in the viral GPCR to destabilize the receptor's inactive state.

PubMed: 25745166
DOI: 10.1126/science.aaa5026

実験手法

X-RAY DIFFRACTION (2.886 Å)