4XOD

Crystal structure of a FimH*DsG complex from E.coli F18

4XOD の概要

• エントリーDOI: 10.2210/pdb4xod/pdb
• 分子名称: FimG protein, FimH protein (3 entities in total)
• 機能のキーワード: type i pilus, catch-bond, cell adhesion, lectin, upec, bacterial adhesin, uti, mannose
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30469.92

構造登録者

Jakob, R.P.,Sauer, M.M.,Glockshuber, R.,Maier, T. (登録日: 2015-01-16, 公開日: 2016-01-27, 最終更新日: 2024-10-16)

主引用文献

Sauer, M.M.,Jakob, R.P.,Eras, J.,Baday, S.,Eris, D.,Navarra, G.,Berneche, S.,Ernst, B.,Maier, T.,Glockshuber, R.
Catch-bond mechanism of the bacterial adhesin FimH.
Nat Commun, 7:10738-10738, 2016

PubMed Abstract: Ligand-receptor interactions that are reinforced by mechanical stress, so-called catch-bonds, play a major role in cell-cell adhesion. They critically contribute to widespread urinary tract infections by pathogenic Escherichia coli strains. These pathogens attach to host epithelia via the adhesin FimH, a two-domain protein at the tip of type I pili recognizing terminal mannoses on epithelial glycoproteins. Here we establish peptide-complemented FimH as a model system for fimbrial FimH function. We reveal a three-state mechanism of FimH catch-bond formation based on crystal structures of all states, kinetic analysis of ligand interaction and molecular dynamics simulations. In the absence of tensile force, the FimH pilin domain allosterically accelerates spontaneous ligand dissociation from the FimH lectin domain by 100,000-fold, resulting in weak affinity. Separation of the FimH domains under stress abolishes allosteric interplay and increases the affinity of the lectin domain. Cell tracking demonstrates that rapid ligand dissociation from FimH supports motility of piliated E. coli on mannosylated surfaces in the absence of shear force.

PubMed: 26948702
DOI: 10.1038/ncomms10738

実験手法

X-RAY DIFFRACTION (1.14 Å)