4XGT

Structure of RNA Helicase FRH a Critical Component of the Neurospora Crassa Circadian Clock

4XGT の概要

• エントリーDOI: 10.2210/pdb4xgt/pdb
• 分子名称: FRQ-interacting RNA helicase (2 entities in total)
• 機能のキーワード: atpase, circadian clock, hydrolase
• 由来する生物種: Neurospora crassa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 112830.94

構造登録者

Conrad, K.S.,Crane, B.C. (登録日: 2015-01-02, 公開日: 2016-07-06, 最終更新日: 2023-09-27)

主引用文献

Conrad, K.S.,Hurley, J.M.,Widom, J.,Ringelberg, C.S.,Loros, J.J.,Dunlap, J.C.,Crane, B.R.
Structure of the frequency-interacting RNA helicase: a protein interaction hub for the circadian clock.
Embo J., 35:1707-1719, 2016

PubMed Abstract: In the Neurospora crassa circadian clock, a protein complex of frequency (FRQ), casein kinase 1a (CK1a), and the FRQ-interacting RNA Helicase (FRH) rhythmically represses gene expression by the white-collar complex (WCC). FRH crystal structures in several conformations and bound to ADP/RNA reveal differences between FRH and the yeast homolog Mtr4 that clarify the distinct role of FRH in the clock. The FRQ-interacting region at the FRH N-terminus has variable structure in the absence of FRQ A known mutation that disrupts circadian rhythms (R806H) resides in a positively charged surface of the KOW domain, far removed from the helicase core. We show that changes to other similarly located residues modulate interactions with the WCC and FRQ A V142G substitution near the N-terminus also alters FRQ and WCC binding to FRH, but produces an unusual short clock period. These data support the assertion that FRH helicase activity does not play an essential role in the clock, but rather FRH acts to mediate contacts among FRQ, CK1a and the WCC through interactions involving its N-terminus and KOW module.

PubMed: 27340124
DOI: 10.15252/embj.201694327

実験手法

X-RAY DIFFRACTION (3.09 Å)