4XGA

Crystal structure of BamB and BamA P3-5 complex from E.coli

4XGA の概要

• エントリーDOI: 10.2210/pdb4xga/pdb
• 分子名称: Outer membrane protein assembly factor BamB, Outer membrane protein assembly factor BamA, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: outer member protein, protein binding-membrane protein complex, protein binding/membrane protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• 細胞内の位置: Cell outer membrane ; Lipid-anchor : P77774; Cell outer membrane : P0A940
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68065.00

構造登録者

Chen, Z.,Zhan, L.H.,Dong, C.,Gao, Z.Q.,Dong, Y.H. (登録日: 2014-12-30, 公開日: 2016-01-20, 最終更新日: 2023-11-08)

主引用文献

Chen, Z.,Zhan, L.H.,Hou, H.F.,Gao, Z.Q.,Xu, J.H.,Dong, C.,Dong, Y.H.
Structural basis for the interaction of BamB with the POTRA3-4 domains of BamA.
Acta Crystallogr D Struct Biol, 72:236-244, 2016

PubMed Abstract: In Escherichia coli, the Omp85 protein BamA and four lipoproteins (BamBCDE) constitute the BAM complex, which is essential for the assembly and insertion of outer membrane proteins into the outer membrane. Here, the crystal structure of BamB in complex with the POTRA3-4 domains of BamA is reported at 2.1 Å resolution. Based on this structure, the POTRA3 domain is associated with BamB via hydrogen-bonding and hydrophobic interactions. Structural and biochemical analysis revealed that the conserved residues Arg77, Glu127, Glu150, Ser167, Leu192, Leu194 and Arg195 of BamB play an essential role in interaction with the POTRA3 domain.

PubMed: 26894671
DOI: 10.1107/S2059798315024729

実験手法

X-RAY DIFFRACTION (2.15 Å)