4XFV

Crystal Structure of Elp2

4XFV の概要

• エントリーDOI: 10.2210/pdb4xfv/pdb
• 分子名称: Elongator complex protein 2 (2 entities in total)
• 機能のキーワード: elp2, elongator complex, translation
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast)
• 細胞内の位置: Cytoplasm: P42935
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 89817.73

構造登録者

Lin, Z.,Dong, C.,Long, J.,Shen, Y. (登録日: 2014-12-29, 公開日: 2015-05-20, 最終更新日: 2023-11-08)

主引用文献

Dong, C.,Lin, Z.,Diao, W.,Li, D.,Chu, X.,Wang, Z.,Zhou, H.,Xie, Z.,Shen, Y.,Long, J.
The elp2 subunit is essential for elongator complex assembly and functional regulation
Structure, 23:1078-1086, 2015

PubMed Abstract: Elongator is a highly conserved multiprotein complex composed of six subunits (Elp1-6). Elongator has been associated with various cellular activities and has attracted clinical attention because of its role in certain neurodegenerative diseases. Here, we present the crystal structure of the Elp2 subunit revealing two seven-bladed WD40 β propellers, and show by structure-guided mutational analyses that the WD40 fold integrity of Elp2 is necessary for its binding to Elp1 and Elp3 subunits in multiple species. The detailed biochemical experiments indicate that Elp2 binds microtubules through its conserved alkaline residues in vitro and in vivo. We find that both the mutually independent Elp2-mediated Elongator assembly and the cytoskeleton association are important for yeast viability. In addition, mutation of Elp2 greatly affects the histone H3 acetylation activity of Elongator in vivo. Our results indicate that Elp2 is a necessary component for functional Elongator and acts as a hub in the formation of various complexes.

PubMed: 25960406
DOI: 10.1016/j.str.2015.03.018

実験手法

X-RAY DIFFRACTION (3.2 Å)