4XDN

Crystal structure of Scc4 in complex with Scc2n

4XDN の概要

• エントリーDOI: 10.2210/pdb4xdn/pdb
• 分子名称: MAU2 chromatid cohesion factor homolog, Sister chromatid cohesion protein 2, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: cohesion, centromere, cohesin loading, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 96905.90

構造登録者

Hinshaw, S.M.,Harrison, S.C. (登録日: 2014-12-19, 公開日: 2015-06-17, 最終更新日: 2024-02-28)

主引用文献

Hinshaw, S.M.,Makrantoni, V.,Kerr, A.,Marston, A.L.,Harrison, S.C.
Structural evidence for Scc4-dependent localization of cohesin loading.
Elife, 4:e06057-e06057, 2015

PubMed Abstract: The cohesin ring holds newly replicated sister chromatids together until their separation at anaphase. Initiation of sister chromatid cohesion depends on a separate complex, Scc2(NIPBL)/Scc4(Mau2) (Scc2/4), which loads cohesin onto DNA and determines its localization across the genome. Proper cohesin loading is essential for cell division, and partial defects cause chromosome missegregation and aberrant transcriptional regulation, leading to severe developmental defects in multicellular organisms. We present here a crystal structure showing the interaction between Scc2 and Scc4. Scc4 is a TPR array that envelops an extended Scc2 peptide. Using budding yeast, we demonstrate that a conserved patch on the surface of Scc4 is required to recruit Scc2/4 to centromeres and to build pericentromeric cohesion. These findings reveal the role of Scc4 in determining the localization of cohesin loading and establish a molecular basis for Scc2/4 recruitment to centromeres.

PubMed: 26038942
DOI: 10.7554/eLife.06057

実験手法

X-RAY DIFFRACTION (2.08 Å)