4XAX
Crystal structure of Thermus thermophilus CarD in complex with the Thermus aquaticus RNA polymerase beta1 domain
Summary for 4XAX
| Entry DOI | 10.2210/pdb4xax/pdb |
| Descriptor | DNA-directed RNA polymerase subunit beta domain 1, CarD, 1,2-ETHANEDIOL, ... (4 entities in total) |
| Functional Keywords | transcription regulator |
| Biological source | Thermus aquaticus More |
| Total number of polymer chains | 2 |
| Total formula weight | 38425.90 |
| Authors | Chen, J.,Bae, B.,Campbell, E.A.,Darst, S.A. (deposition date: 2014-12-15, release date: 2015-09-16, Last modification date: 2023-09-27) |
| Primary citation | Bae, B.,Chen, J.,Davis, E.,Leon, K.,Darst, S.A.,Campbell, E.A. CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex. Elife, 4:-, 2015 Cited by PubMed Abstract: A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli. To delineate the molecular mechanism of CarD, we determined crystal structures of Thermus transcription initiation complexes containing CarD. The structures show CarD interacts with the unique DNA topology presented by the upstream double-stranded/single-stranded DNA junction of the transcription bubble. We confirm that our structures correspond to functional activation complexes, and extend our understanding of the role of a conserved CarD Trp residue that serves as a minor groove wedge, preventing collapse of the transcription bubble to stabilize the transcription initiation complex. Unlike E. coli RNAP, many bacterial RNAPs form unstable promoter complexes, explaining the need for CarD. PubMed: 26349034DOI: 10.7554/eLife.08505 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.404 Å) |
Structure validation
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