4XAX

Crystal structure of Thermus thermophilus CarD in complex with the Thermus aquaticus RNA polymerase beta1 domain

4XAX の概要

• エントリーDOI: 10.2210/pdb4xax/pdb
• 分子名称: DNA-directed RNA polymerase subunit beta domain 1, CarD, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: transcription regulator
• 由来する生物種: Thermus aquaticus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38425.90

構造登録者

Chen, J.,Bae, B.,Campbell, E.A.,Darst, S.A. (登録日: 2014-12-15, 公開日: 2015-09-16, 最終更新日: 2023-09-27)

主引用文献

Bae, B.,Chen, J.,Davis, E.,Leon, K.,Darst, S.A.,Campbell, E.A.
CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.
Elife, 4:-, 2015

PubMed Abstract: A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli. To delineate the molecular mechanism of CarD, we determined crystal structures of Thermus transcription initiation complexes containing CarD. The structures show CarD interacts with the unique DNA topology presented by the upstream double-stranded/single-stranded DNA junction of the transcription bubble. We confirm that our structures correspond to functional activation complexes, and extend our understanding of the role of a conserved CarD Trp residue that serves as a minor groove wedge, preventing collapse of the transcription bubble to stabilize the transcription initiation complex. Unlike E. coli RNAP, many bacterial RNAPs form unstable promoter complexes, explaining the need for CarD.

PubMed: 26349034
DOI: 10.7554/eLife.08505

実験手法

X-RAY DIFFRACTION (2.404 Å)