4X8J

Crystal Structure of murine 12F4 Fab monoclonal antibody against ADAMTS5

Summary for 4X8J

Entry DOI	10.2210/pdb4x8j/pdb
Related	4X80
Descriptor	12F4 FAB Light chain, 12F4 FAB Heavy chain, ZINC ION, ... (5 entities in total)
Functional Keywords	monoclonal, immune system
Biological source	Mus musculus More
Total number of polymer chains	4
Total formula weight	95543.12
Authors	Larkin, J.,Lohr, T.A.,Elefante, L.,Shearin, J.,Matico, R.,Su, J.-L.,Xue, Y.,Liu, F.,Genell, C.,Miller, R.E.,Tran, P.B.,Malfait, A.-M.,Maier, C.C.,Matheny, C.J. (deposition date: 2014-12-10, release date: 2015-04-08, Last modification date: 2024-10-09)
Primary citation	Larkin, J.,Lohr, T.A.,Elefante, L.,Shearin, J.,Matico, R.,Su, J.L.,Xue, Y.,Liu, F.,Genell, C.,Miller, R.E.,Tran, P.B.,Malfait, A.M.,Maier, C.C.,Matheny, C.J. Translational development of an ADAMTS-5 antibody for osteoarthritis disease modification. Osteoarthr. Cartil., 23:1254-1266, 2015 Cited by PubMed Abstract: Aggrecanase activity, most notably ADAMTS-5, is implicated in pathogenic cartilage degradation. Selective monoclonal antibodies (mAbs) to both ADAMTS-5 and ADAMTS-4 were generated and in vitro, ex vivo and in vivo systems were utilized to assess target engagement, aggrecanase inhibition and modulation of disease-related endpoints with the intent of selecting a candidate for clinical development in osteoarthritis (OA). PubMed: 25800415 DOI: 10.1016/j.joca.2015.02.778 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.35 Å)

Structure validation