4WZG

Structure of human ATG101

Summary for 4WZG

• Entry DOI: 10.2210/pdb4wzg/pdb
• Descriptor: Autophagy-related protein 101, BETA-MERCAPTOETHANOL (3 entities in total)
• Functional Keywords: autophagy, ulk1 complex, horma domain, atg13 binding, protein binding
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm : Q9BSB4
• Total number of polymer chains: 1
• Total formula weight: 25114.80

Authors

Michel, M.,Weiergraeber, O.H. (deposition date: 2014-11-19, release date: 2015-06-17, Last modification date: 2024-04-03)

Primary citation

Michel, M.,Schwarten, M.,Decker, C.,Nagel-Steger, L.,Willbold, D.,Weiergraber, O.H.
The mammalian autophagy initiator complex contains 2 HORMA domain proteins.
Autophagy, 11:2300-2308, 2015

PubMed Abstract: ATG101 is an essential component of the ULK complex responsible for initiating cellular autophagy in mammalian cells; its 3-dimensional structure and molecular function, however, are currently unclear. Here we present the X-ray structure of human ATG101. The protein displays an open HORMA domain fold. Both structural properties and biophysical evidence indicate that ATG101 is locked in this conformation, in contrast to the prototypical HORMA domain protein MAD2. Moreover, we discuss a potential mode of dimerization with ATG13 as a fundamental aspect of ATG101 function.

PubMed: 26236954
DOI: 10.1080/15548627.2015.1076605

Experimental method

X-RAY DIFFRACTION (1.9 Å)