4WVO

An engineered PYR1 mandipropamid receptor in complex with mandipropamid and HAB1

4WVO の概要

• エントリーDOI: 10.2210/pdb4wvo/pdb
• 分子名称: Abscisic acid receptor PYR1, Protein phosphatase 2C 16, (2S)-2-(4-chlorophenyl)-N-{2-[3-methoxy-4-(prop-2-yn-1-yloxy)phenyl]ethyl}-2-(prop-2-yn-1-yloxy)ethanamide, ... (5 entities in total)
• 機能のキーワード: pyr/pyl/rcar, pyr1, hab1, mandipropamid, pp2c inhibitor, hydrolase-hydrolase inthibitor complex, hydrolase/hydrolase inthibitor
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• 細胞内の位置: Cytoplasm : O49686 Q9CAJ0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57727.02

構造登録者

Peterson, F.C.,Volkman, B.F.,Cutler, S.R. (登録日: 2014-11-06, 公開日: 2015-02-11, 最終更新日: 2023-10-18)

主引用文献

Park, S.Y.,Peterson, F.C.,Mosquna, A.,Yao, J.,Volkman, B.F.,Cutler, S.R.
Agrochemical control of plant water use using engineered abscisic acid receptors.
Nature, 520:545-548, 2015

PubMed Abstract: Rising temperatures and lessening fresh water supplies are threatening agricultural productivity and have motivated efforts to improve plant water use and drought tolerance. During water deficit, plants produce elevated levels of abscisic acid (ABA), which improves water consumption and stress tolerance by controlling guard cell aperture and other protective responses. One attractive strategy for controlling water use is to develop compounds that activate ABA receptors, but agonists approved for use have yet to be developed. In principle, an engineered ABA receptor that can be activated by an existing agrochemical could achieve this goal. Here we describe a variant of the ABA receptor PYRABACTIN RESISTANCE 1 (PYR1) that possesses nanomolar sensitivity to the agrochemical mandipropamid and demonstrate its efficacy for controlling ABA responses and drought tolerance in transgenic plants. Furthermore, crystallographic studies provide a mechanistic basis for its activity and demonstrate the relative ease with which the PYR1 ligand-binding pocket can be altered to accommodate new ligands. Thus, we have successfully repurposed an agrochemical for a new application using receptor engineering. We anticipate that this strategy will be applied to other plant receptors and represents a new avenue for crop improvement.

PubMed: 25652827
DOI: 10.1038/nature14123

実験手法

X-RAY DIFFRACTION (2.251 Å)