4WUJ

Structural Biochemistry of a Fungal LOV Domain Photoreceptor Reveals an Evolutionarily Conserved Pathway Integrating Blue-Light and Oxidative Stress

4WUJ の概要

• エントリーDOI: 10.2210/pdb4wuj/pdb
• 分子名称: Glycoside hydrolase family 15, cellulose signaling associated protein envoy, FLAVIN MONONUCLEOTIDE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: lov domain, blue-light, photoreceptor, circadian clock, circadian clock protein
• 由来する生物種: Hypocrea jecorina
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 66234.99

構造登録者

Hopkins, H.C.,Lokhandwala, J.,Zoltowski, B.D. (登録日: 2014-10-31, 公開日: 2015-01-14, 最終更新日: 2023-09-27)

主引用文献

Lokhandwala, J.,Hopkins, H.C.,Rodriguez-Iglesias, A.,Dattenbock, C.,Schmoll, M.,Zoltowski, B.D.
Structural Biochemistry of a Fungal LOV Domain Photoreceptor Reveals an Evolutionarily Conserved Pathway Integrating Light and Oxidative Stress.
Structure, 23:116-125, 2015

PubMed Abstract: Fungal LOV proteins facilitate photoadaptation via blue light regulation of dimer formation. Despite considerable homology of these proteins in closely related fungi, deviations in signaling exist. Here we report the crystal structure of ENVOY (ENV1), a homolog of N. crassa VVD in the fungus T. reesei, a model organism for plant cell wall degradation. Structural studies contradict a model of reversible competitive dimerization. Rather, evolutionary pressures have facilitated a two-residue shift in the position of a key Cys residue (Cys96) that enables the integration of environmental stress and light responses. A Cys96Thr variant abolishes adaptive responses to light and oxidative stress in a carbon source-dependent manner in vivo. Phylogenetic analysis verifies an evolutionary relevance of the Cys residue shift in different orders within Sordariomycetes. In this manner, we identified a widespread oxidative stress signaling mechanism that couples metabolic sensing and blue light responses not previously identified in LOV proteins.

PubMed: 25533487
DOI: 10.1016/j.str.2014.10.020

実験手法

X-RAY DIFFRACTION (2.23 Å)