4WSM

Complex of 70S ribosome with tRNA-Leu and mRNA with G-U mismatch in the first position in the A- and P-sites

これはPDB形式変換不可エントリーです。

4WSM の概要

• エントリーDOI: 10.2210/pdb4wsm/pdb
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (57 entities in total)
• 機能のキーワード: ribosome, translation, mismatch
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 108
• 化学式量合計: 4555498.71

構造登録者

Rozov, A.,Demeshkina, N.,Yusupov, M.,Yusupova, G. (登録日: 2014-10-28, 公開日: 2015-06-10, 最終更新日: 2024-01-10)

主引用文献

Rozov, A.,Demeshkina, N.,Westhof, E.,Yusupov, M.,Yusupova, G.
Structural insights into the translational infidelity mechanism.
Nat Commun, 6:7251-7251, 2015

PubMed Abstract: The decoding of mRNA on the ribosome is the least accurate process during genetic information transfer. Here we propose a unified decoding mechanism based on 11 high-resolution X-ray structures of the 70S ribosome that explains the occurrence of missense errors during translation. We determined ribosome structures in rare states where incorrect tRNAs were incorporated into the peptidyl-tRNA-binding site. These structures show that in the codon-anticodon duplex, a G·U mismatch adopts the Watson-Crick geometry, indicating a shift in the tautomeric equilibrium or ionization of the nucleobase. Additional structures with mismatches in the 70S decoding centre show that the binding of any tRNA induces identical rearrangements in the centre, which favours either isosteric or close to the Watson-Crick geometry codon-anticodon pairs. Overall, the results suggest that a mismatch escapes discrimination by preserving the shape of a Watson-Crick pair and indicate that geometric selection via tautomerism or ionization dominates the translational infidelity mechanism.

PubMed: 26037619
DOI: 10.1038/ncomms8251

実験手法

X-RAY DIFFRACTION (3.3 Å)