4WR4

Crystal Structure of GST Mutated with Halogenated Tyrosine (7bGST-1)

4WR4 の概要

• エントリーDOI: 10.2210/pdb4wr4/pdb
• 関連するPDBエントリー: 4WR5
• 分子名称: Glutathione S-transferase class-mu 26 kDa isozyme, GLUTATHIONE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: themostabilized structure, homodimeric, two domains, alfa/beta, alfa, detoxification, xenobiotic compounds, gutathione, 7 specfic sites, cytoplasmic, transferase
• 由来する生物種: Schistosoma japonicum (Blood fluke)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29187.21

構造登録者

Akasaka, R.,Kawazoe, M.,Tomabechi, Y.,Ohtake, K.,Itagaki, T.,Takemoto, C.,Shirouzu, M.,Yokoyama, S.,Sakamoto, K. (登録日: 2014-10-23, 公開日: 2015-08-19, 最終更新日: 2023-11-08)

主引用文献

Ohtake, K.,Yamaguchi, A.,Mukai, T.,Kashimura, H.,Hirano, N.,Haruki, M.,Kohashi, S.,Yamagishi, K.,Murayama, K.,Tomabechi, Y.,Itagaki, T.,Akasaka, R.,Kawazoe, M.,Takemoto, C.,Shirouzu, M.,Yokoyama, S.,Sakamoto, K.
Protein stabilization utilizing a redefined codon
Sci Rep, 5:9762-9762, 2015

PubMed Abstract: Recent advances have fundamentally changed the ways in which synthetic amino acids are incorporated into proteins, enabling their efficient and multiple-site incorporation, in addition to the 20 canonical amino acids. This development provides opportunities for fresh approaches toward addressing fundamental problems in bioengineering. In the present study, we showed that the structural stability of proteins can be enhanced by integrating bulky halogenated amino acids at multiple selected sites. Glutathione S-transferase was thus stabilized significantly (by 5.2 and 5.6 kcal/mol) with 3-chloro- and 3-bromo-l-tyrosines, respectively, incorporated at seven selected sites. X-ray crystallographic analyses revealed that the bulky halogen moieties filled internal spaces within the molecules, and formed non-canonical stabilizing interactions with the neighboring residues. This new mechanism for protein stabilization is quite simple and applicable to a wide range of proteins, as demonstrated by the rapid stabilization of the industrially relevant azoreductase.

PubMed: 25985257
DOI: 10.1038/srep09762

実験手法

X-RAY DIFFRACTION (1.6 Å)