4WNA

Structure of the Nitrogenase MoFe Protein from Azotobacter vinelandii Pressurized with Xenon

4WNA の概要

• エントリーDOI: 10.2210/pdb4wna/pdb
• 関連するPDBエントリー: 4WN9
• 分子名称: Nitrogenase molybdenum-iron protein alpha chain, Nitrogenase molybdenum-iron protein beta chain, 3-HYDROXY-3-CARBOXY-ADIPIC ACID, ... (8 entities in total)
• 機能のキーワード: xenon, mofe protein, nitrogenase, substrate access, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Azotobacter vinelandii; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 234026.92

構造登録者

Morrison, C.N.,Hoy, J.A.,Zhang, L.,Einsle, O.,Rees, D.C. (登録日: 2014-10-11, 公開日: 2015-03-18, 最終更新日: 2023-12-27)

主引用文献

Morrison, C.N.,Hoy, J.A.,Zhang, L.,Einsle, O.,Rees, D.C.
Substrate Pathways in the Nitrogenase MoFe Protein by Experimental Identification of Small Molecule Binding Sites.
Biochemistry, 54:2052-2060, 2015

PubMed Abstract: In the nitrogenase molybdenum-iron (MoFe) protein, we have identified five potential substrate access pathways from the protein surface to the FeMo-cofactor (the active site) or the P-cluster using experimental structures of Xe pressurized into MoFe protein crystals from Azotobacter vinelandii and Clostridium pasteurianum. Additionally, all published structures of the MoFe protein, including those from Klebsiella pneumoniae, were analyzed for the presence of nonwater, small molecules bound to the protein interior. Each pathway is based on identification of plausible routes from buried small molecule binding sites to both the protein surface and a metallocluster. Of these five pathways, two have been previously suggested as substrate access pathways. While the small molecule binding sites are not conserved among the three species of MoFe protein, residues lining the pathways are generally conserved, indicating that the proposed pathways may be accessible in all three species. These observations imply that there is unlikely a unique pathway utilized for substrate access from the protein surface to the active site; however, there may be preferred pathways such as those described here.

PubMed: 25710326
DOI: 10.1021/bi501313k

実験手法

X-RAY DIFFRACTION (2 Å)